Terminator

Part:BBa_K3385005:Design

Designed by: Daniel Bavnh�j   Group: iGEM20_DTU-Denmark   (2020-10-12)


Aspergillus fumigatus U3 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 97
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 97
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 97
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 97
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is well known and is known to work and can be cloned using several cloning methods, such as USER and Gibson.

Source

Obtained as part of the CRISPR cloning vector pFC902[1]. It originates from A. fumigatus.

pFC902 was a gift from Uffe Mortensen (Addgene plasmid # 106904 ; http://n2t.net/addgene:106904 ; RRID:Addgene_106904)

References

[1] Efficient Oligo nucleotide mediated CRISPR-Cas9 Gene Editing in Aspergilli. Nodvig CS, Hoof JB, Kogle ME, Jarczynska ZD, Lehmbeck J, Klitgaard DK, Mortensen UH. Fungal Genet Biol. 2018 Jan 8. pii: S1087-1845(18)30004-5. doi: 10.1016/j.fgb.2018.01.004. 10.1016/j.fgb.2018.01.004 PubMed 29325827